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BD 固定/透化试剂盒554714,
BD Cytofix/Cytoperm™ Fixation/Permeabilization Kit
本试剂盒用于细胞固定和破膜,以便使用荧光标记的抗细胞因子抗体对胞内细胞因子进行染色。
试剂盒组成
固定/破膜液(Fixation/Permeabilization Solution)
BD Perm/Wash™ 缓冲液
操作流程
细胞固定与破膜:使用固定/破膜液处理细胞。
洗涤与抗体稀释:采用 BD Perm/Wash™ 缓冲液 清洗细胞,并稀释抗细胞因子抗体用于染色。
This kit enables the fixation and permeabilization of cells which is necessary for staining intracellular cytokines with fluorochrome-conjugated anti-cytokine antibodies. The kit provides two reagents, fixation/permeabilization solution and BD Perm/Wash™ Buffer. After cell fixation and permeabilization, the BD Perm/Wash™ Buffer is used to wash the cells and to dilute the anti-cytokine antibodies for staining.
BD 固定/透化试剂盒554714,BD Cytofix/Cytoperm™ Fixation/Permeabilization Kit